Tải bản đầy đủ - 0 (trang)
16 β-Oxidation of Fatty Acids

16 β-Oxidation of Fatty Acids

Tải bản đầy đủ - 0trang


Animal Physiology


Fatty acid

R – R – CH2 – CH2 –C–OH








Acyl – CoA


(Active fatty acid) R – CH – CH – C~S – CoA




Acyl – CoA



Fp H2



O Respiratory chain

A b – Unsaturated R – CH = CH – C~S – CoA

Acyl – CoA








R–CH–CH2–C~S – CoA

acyl – CoA

3~ P




CoA dehydrogenase




Respiratory chain




R–C–CH2–C~S – CoA



b– Ketothiolase





R–C~S–CoA + CH3–C~S – CoA







Fig. 6.15


The acetate molecules can be completely oxidised via citric acid cycle or may be utilised to synthesise

glucose and other complex carbohydrates as per needs of the animal. Some important steps of boxidation scheme are shown in Fig. 6.15.

It must be borne in mind that fatty acid oxidation takes place in the mitochondria, but before FA

enters the mitochondria it has to be made ready for oxidation reactions. The FA in the cytosol is

activated by a molecule of ATP in the presence of acylcoenzyme A(CoASH). The reaction occurs

either in the endoplasmic reticulum or at the outer mitrochondrial membrane, resulting in the

formation of fatty acyl CoA derivative. The fatty acyl CoA derivative is then transported inside the

mitochondria with the help of carnitine, a carrier molecule. This reaction is catalysed by an enzyme,

acyl CoA transferase. Once the fatty acyl CoA enters the mitochondrial matrix, there follows the

removal of 2 hydrogen atoms from the = and b carbons, catalysed by a dehydrogenase, resulting in



the formation of unsaturated acyl CoA. The unsaturated fatty acyl CoA derivative is subsequently

hydrated and dehydrogenated at the expense of specific enzymes to form corresponding b-keto-acyl

CoA compound. Finally, b-keto-acyl CoA undergoes thiolytic cleavage by thiolase producing an acylCoA unit and the remaining acyl-CoA chain containing 2-C less than the original fatty acyl CoA

molecule. In this way, a long chain fatty acid may be degraded completely to acetyl-CoA (2-C

fragments), which can be oxidised to CO2 and water through citric acid cycle.

In case of fatty acids with odd number of carbon atoms, oxidation takes place through b-oxidation

scheme, leaving behind propionyl CoA, a 3-carbon unit. This compound can enter the citric acid cycle

after conversion to succinyl CoA.

Energetics of >-oxidation

Let us consider the oxidation of one mole of palmitic acid (C16 H32 O2), entering the mitochondria in

the form of palmitoyl CoA. Initially one mole of ATP is required to activate the acid, and at the end of

each oxidative spiral, one FADH2 and one NADH are formed along with an acetyl CoA fragment. In

order to oxidise palmitoyl CoA, 8 acetyl CoA will be formed and the energy gained in terms of ATP

will be as follows:

8 acetyl CoA + 7 FADH2 + 7 NADH + H+

8 acetyl CoA oxidised via citric acid cycle

ATP initially used for activation

Net gain of ATP

35 ATP formed

96 ATP formed

01 ATP consumed

130 ATP

The overall equation is represented as:

C16 H32 O2 + ATP + 7 FAD + 7 NAD+ + 8 CoASH + 7 H2O ® AMP

+ PPi + 7 FADH2 + 7 NADH + H+ + 8 CH3CO—S—CoA.

Since each ATP molecule has 7.6 kcal of bond energy, the net gain would be 130 ´ 7.6 = 988

kilocalories. The calorific value of palmitic acid is 2340 kcal/mole, the system receives at least 42%

of high phosphate bond energy (988/2340 ´ 100) of the total energy of combustion of the fatty acid.

Oxidation of Unsaturated Fatty Acids

Body lipids are rich in unsaturated fatty acids and these are oxidised more slowly. Some examples are:

palmitoleic acid (16:1), oleic acid (18:1), linoleic acid (18:2), linolenic acid (18:3) and arachidonic

acid (20:4). All double bonds in naturally occurring unsaturated fatty acids are in cis-configuration.

Palmitoleic and oleic acids are not essential as they can be formed in the body, but the remaining three

acids come under the essential fatty acids category and have to be supplied in the diet. Oxidation of

unsaturated fatty acids proceeds the usual b-oxidative pathway until the double bond is reached. The

double bond in cis-configuration is not vulnerable to enzymic attack unless it is isomerised to transconfiguration. Polyunsaturated fatty acids, such as linoleic, arachidonic etc. are more complex and

require additional enzyme for oxidation. They are normally found as structural components in

association with cholesterol and phospholipids (e.g. membranes and reproductive organs). In

mammals, arachidonic and some related C-20 fatty acids are known to give rise to unique compounds

like prostaglandins which have hormone-like activity.


Animal Physiology


One of the hydrolysis products of triglycerides is glycerol which is metabolised or utilised in

organs/tissues where specific enzyme glycerol kinase is abundantly present. Certain organs such as

liver, kidney, intestinal mucosa and lactating mammary glands are rich in the enzyme, while muscles

and adipose tissue contain very little activity. Glycerol is predominantly converted into carbohydrate

through glycerol phosphate, formed by a specific glycerol kinase at the expanse of ATP.








Glycerol phosphate

(triose phosphate)

Glycerol phosphate is then oxidised to triosephosphate by a glycerol phosphate dehydrogenase

which ultimately forms glycogen through glycogenesis. Triosephosphate may be, however, oxidised

to pyruvic acid by way of glycolysis (Fig. 6.16). In diabetic or phlorrhizinised animals, glycerol is

converted almost quantitatively to glucose.

Fig. 6.16

Fate of glycerol metabolism.


It has been known for a long time that fats are synthesised from metabolites such as acetate and

acetoacetate. Fats are also synthesised from protein and carbohydrate sources. All naturally occurring

fatty acids possess even number of carbon atoms; thus it is logical that fatty acids must be synthesised

from 2-carbon fragments. It has been shown that the starting material is acetyl-CoA which can be

derived from pyruvate. Alternatively, pyruvate can also be derived from free, acetate when it reacts

with coenzyme A and ATP.

Synthesis of Glycerides

Triglycerides are formed by reactions between acyl-CoA compounds and µ-glycerophosphate, which

is formed by specific glycerol kinase. There are other enzymes which catalyse the formation of

mono—and diphosphatidic acids at the expense of fatty acyl CoA derivatives:



CH2 O—R1






Phosphatidic acid

In the next stage the phosphate group is removed by a specific phosphatase followed by

replacement by a third fatty acyl residue:






Phosphatidic acid

CH2 O—R1

CH2 O—R1

CH2 O—R1





+ Acyl CoA




Synthesis of Fatty Acids

Fatty acid oxidation occurs in the mitochondria, but fatty acid synthesis takes place not only in

mitochondria but also in mitochondria-free systems. The pathway for synthesis is not exactly reversal

of b-oxidation scheme, but it involves, some modifications. Under anaerobic conditions, mitochondria

catalyse the incorporation of acetyl-CoA units into long chain fatty acids (viz. stearic acid, palmitic

acid), requiring ATP, NADH and NADPH. Synthesis in extra-mitochondrial system, especially in the

liver, brain, kidney etc., acetyl-CoA units are incorporated into fatty acids, catalysed by cytosolic

enzymes and cofactors such as ATP, NADPH and Mg2+ or Mn2+ ions. This system is dependent on

CO2 supplied by bicarbonate. Chain elongation usually takes place in the microsomes.


Phospholipids are found in all cells and are synthesised either from phosphatidic acid or phosphatidyl

choline. Lecithin is the most important phospholipid in the body and is synthesised in the liver.

Synthesis of phospholipids from fats involves mobilisation of fats in and out of the cells. Hence the

actual sites are liver, intestine and kidney. Phospholipids are largely found in combination with

proteins and are transported in the blood in the form of protein complexes.

The most common among phospholipids are lecithins and cephalins, while where are others in

which bases are replaced by serine or inositol. Catabolism of lecithin is accomplished in the following

manner (Fig. 6.17):

Sphingomyelins are phospholipids containing a fatty acid, phosphoric acid, choline, and a

complex of amino alcohol, sphingosine, but are devoid of glycerol. Abnormal quantitities of phosphoand sphingolipids in certain tissues, especially in the nervous system, cause diseases like

sphingolipidoses, and demyleinating diseases that are inherited.


Cholesterol occurs in various body tissues and in the plasma either in the free form or storage form as

long chain fatty acid esters belonging to the class of sterols. It is also an important component of the


Animal Physiology



Phospholipase A




Glyceryephosphoryl choline


choline esterase

a - Glycerophosphate + Choline

Fig. 6.17

Catabolism of lecithin.

membrane system of the cell and is the precursor of steroid hormones, excreted from the body in the

form of bile acids (salts). The cholesterol content of the blood ranges from 150 – 250 mg/100 ml.

Sources of Cholesterol in the Body

While major part of the body cholesterol (about 1 g/day) is synthesized in the body, a small portion is

(about 0.3 g/day) provided by various foods intake such as egg yolk, meat, liver, and brain and its

uptake is through low density lipoproteins (LDLs). Synthesis occurs from acetyl-CoA (two-carbon

units) produced by beta oxidation of fatty acids in the liver, intestine and almost all cells. About 700

mg/d is synthesized by a well regulated mechanism. Free cholesterol is removed from the body by

plasma high-density lipoproteins (HDLs).

Synthesis of Cholesterol

Although cholesterol is synthesized by many tissues such as adrenal cortex, skin, intestine testes etc.

liver is considered to be the main site. Two molecules of acetyl-CoA condense to form acetoacetylCoA, which again reacts with another molecule of acetyl-CoA to form 3-hydroxy-3-methylglutarylCoA (HMG-CoA), leading to the synthesis of mevalonate (Fig. 6.18). It must be noted that all carbon

atoms of cholesterol originate from acetyl-CoA enzyme. Mevalonate is the crucial compound and

through a series of reactions, gives rise to cholesterol.

Transport and Excretion of Cholesterol

Cholesterol, in association with other lipids, is absorbed in the intestine and thereafter incorporated

into chylomicrons and very low-density lipoproteins (VLDLs). A greater part of cholesterol (about

80%) in the lymph undergoes esterification with long-chain fatty acids and transported as lipoproteins

in the plasma. Highest proportion of cholesterol is found in the LDL. Free cholesterol in the plasma is



2 CH3–CO–S–CoA

(Acetyl CoA)



(Acetoacetyl CoA)






(3-Hydroxy-3-methylgutaryl CoA)




CoA HMG CoA reductase



(Mevalonic Acid)

To synthesis of cholesterol

Fig. 6.18

Steps in the synthesis of mevalonic acid, the precursor of cholesterol synthesis (steps not shown).

equilibrated with the cholesterol esters and about half of the free cholesterol is eliminated by

excretion in the faeces, while remainder is excreted as neutral steroid.

The liver contains a pool of unesterified cholesterol from where it is transported to the intestine

and then back to liver through chylomicrons. Cholesterol is excreted from the body as cholesterol or

bile acids. Coprostanol is the main sterol secreted in the faeces, a product of cholesterol, arising from

the intestinal bacteria activity. Much of the cholesterol secreted in the bile is either reabsorbed or gets

deposited in the arteries in the form of esters or may participate in steroid synthesis.


During conditions of starvation and diabetes, oxidation of free fatty acids is enhanced in the

mitochondria. In vertebrates, the acetyl-CoA originating from the b-oxidation can leave the

mitochondrion in the form of citrate. However, a major part of this acetyl-CoA is used for the

synthesis of ketone bodies in the liver (ketosis). There are three types of ketone bodies: acetone,

acetoacetic acid, and 3-hydroxybutyric acid, which are generated through a process called

ketogenesis, taking place in the mitochondria.

Formation of Ketone Bodies

Long-chain fatty acid are transported to the inner mitochondrial membrane through carnitine

derivatives. b-oxidation of fatty acids takes place in the mitochondrion, releasing acetyl-CoA, with


Animal Physiology

the consequent production of large amount of ATP. The initial steps are similar to those of cholesterol

synthesis (Fig. 6.19). The 3-hydroxy-3-methylglutaryl-CoA formed is split into acetyl-CoA and free

acetoacetic acid.

The acetoacetic acid is either spontaneously decarboxylated to acetone or reduced to 3hydroxybutyric acid:


(Acetoacetic acid)

CO2 + CH3–CO–CH3





(3-Hydroxybutyric acid)


Long-chain fatty acids (even number) produce acetyl-CoA units through beta oxidation, but odd

chain fatty acids produce acetyl-CoA and propionyl-CoA, which is glucogenic. Peroxisomes oxidize

long-chain fatty acids.

Ketone Bodies Serve as Energy Source

Acetoacetic acid and 3-hydroxybutyric acid are produced in large amount and are utilized as energy

source by various tissues such as muscles, kidney and the brain (extrahepatic tissues). Acetoacetic

acid diffuses freely across the cell membranes and cannot be reactivated unless they reach the cytosol

where they can participate in cholesterol synthesis as happens with acetoacetate.

Acetoacetate is reactivated in extrahepatic tissues:

Acetoacetate + Succinyl-CoA ® Succinic acid + Acetoacetyl-CoA. Acetoacetyl-CoA splits by

thiolysis into two molecules of acetyl-CoA which enters the Krebs cycle. Ketogenesis is an extremely

important physiological process and is exclusively hepatic.

Ketogenesis is regulated chiefly by two hormones: insulin and glucagon. When blood glucose

levels are decreased, insulin is depressed, thereby raising glucagon levels, resulting in arresting

glycolysis, increase in gluconeogenesis and inhibiting fatty acid synthesis. As a consequence,

hydrolysis of triglycerides is affected by hormone-dependent lipase in adipose tissues. The fatty acids

thus liberated are transported to the liver.

The hormonal imbalance inhibits acetyl-CoA carboxylase, depressing malonyl-CoA

concentration, which is a repressor of acetylcarnitine synthetase, hence it gets activated. This favours

penetration of fatty acids into the mitochondria where they are catabolized by beta oxidation,

increasing the NADH/NAD ratio. This will lead to reduction of oxaloacetate to malate which leaves

mitochondria to participate in gluconeogenesis. The reduction of oxaloacetate prevents transformation

into citrate of acetyl-CoA arising from b-oxidation. Thus the only fate of acetyl-CoA is to synthesize

ketone bodies to be transported to other tissues capable of utilizing them.

Oxidation of ketone bodies takes place in the extrahepatic tissues because of the absence of key

enzymes in the liver to oxidize them, i.e. 3-oxoacid-CoA transferase. This enzyme is present in the

kidney, red muscle, brain and other peripheral tissues. Normally ketone bodies are continuously

oxidized in muscles, therefore only traces of these may be found in the blood and urine. In fasting

mammals these accumulate in blood and the levels may rise to 20-25 mg/100 ml after a week’s

fasting. 3-hydroxybutyrate apearing in the blood reaches the muscles (skeletal as well as the cardiac),

where a mitochondrial enzyme, 3-hydroxybutyrate dehydrogenase oxidizes the compound to



acetoacetic acid. The latter compound in the muscle mitochondria is activated by 3-oxoacid-CoA

transferase, catalyzing the transfer of CoA from succinyl-CoA to acetoacetic acid. Excessive

production of ketone bodies by the liver leads to ketonemia.

2 CH3–CO–S–CoA

(2 Acetyl CoA)



(Acetoacetyl CoA)

H2O + Acetyl CoA





(3-Hydroxy-3-methylglutaryl CoA)

Acetyl CoA












Fig. 6.19

Formation of ketone bodies from acetyl CoA.





Digestion and


In the previous chapter, we have given an account of the classification and nature of nutrients required

by animals. The food materials available to the animals are, as such, rarely in a form suitable for

cellular consumption and to achieved when the foods are subjected to mechanical processes such as

mastication, swallowing and movements of gastrointestinal tract, and chemical processes such as the

enzymatic reactions in the digestive tract.

During the process of digestion, the complex nutrients are split into their simpler substances, i.e.

the proteins into amino acids; the polysaccharides into monosaccharides; and the fats into their

constituent fatty acids and glycerols. In addition to hydrolysis of proteins, carbohydrates and fats, the

process of digestion renders food in a soluble form for abosrption and helps in separating the required

from those not required.


Based on their method of food procurement the living organisms (plants, animals and

microorganisms) are broadly classified into two major groups, viz. autotrophs and heterotrophs.


The autotrophs synthesize all essential organic compounds from inorganic constituents. They include

phototrophs and chemotrophs. The phototrophs are chlorophyll bearing plants and make the essential

organic compounds by photosynthesis. For this process they require sunlight, and inorganic

substances like carbon dioxide and nitrogenous compounds. The chemotrophs are bacteria making

their food by chemo-synthesis. Since the autotrophs synthesize the required organic substances within

their body, digestion is not required.

Digestion and Absorption



The heterotrophs require organic substances as food and their synthesizing capability is limited. For

this reason they depend on organic substances like carbohydrates, fats and proteins to carry out their

life processes. They obtain their food from plants and animals. Such a food is in a form unsuitable for

direct absorption. The food may be in the form of tiny particles (food of protozoans and other lower

organisms), large chunks or whole animals (as in higher animals), or in the liquid form (leeches,

certain insects, etc.). Therefore these food particles are to be reduced to sizes suitable for entrance

through the cellular membranes to take part in metabolic activities. This process as we have

mentioned earlier is called digestion and only in heterotrophs it is a necessary process with the

exception of certain parasites (cestodes, etc.) and commensals. The cestodes directly absorb the

digested food through its body surface, from its surrounding medium in the intestine. The male

echiurid derives its nourishment from the female echiurid. Such organisms are devoid of a digestive

tract of their own.

The organisms are thus broadly divided into two groups. However, there are living beings

between the typical chemotrophs and the typical phototrophs, and also between the autotrophs and the

heterotrophs. Such living beings are dependent on both the processes and hence are grouped under

mesotrophs. The Euglena, for example, is a mesotroph and can synthesize essential organic

compounds but it still requires certain growth factors or vitamins for which it is dependent on organic



In order to capture different kinds of food and prepare it for absorption by the cells of the body, the

heterotrophs have suitable anatomical structures and devices.

Among animals the feeding mechanisms exhibit some relation to the type of food they take. It is

reasonable to presume that food of the most primitive organisms is organic matter in solution.

Similarly organic matter in dissolved form is directly absorbed by protozoan parasites, tapeworms and

a few other animals. Studies on the absorption of dissolved organic substances in bacteria as well as

in the cells of higher animals revealed the existence of a number of active sites, each of which

receives the specific compounds to lead them into the cell. Dissolved substances are absorbed by yet

another process. In this process minute droplets of dissolved food are engulfed by the cell through


Protozoans prey upon parts of other organisms smaller than or equal to themselves. To ingest this

type of food the protozoans have a variety of mechanisms. Amoeba, for example, has a mechanism

which may possibly be an extension of pinocytosis and upon coming in contact with its food particle

the amoeba surrounds the particle and engulfs it. Another distinct mechanism of feeding can be

observed among ciliates. The ciliates produce water currents by ciliary movements and these currents

carry the impaled organisms or the particulate food to the mouth or gullet. From here the food is taken

into the body. In sponges the method of collecting food particles by water currents and their

subsequent engulfing by cells for digestion exists in a specialized form. In this method the food

particles are carried along the water currents into the paragastric cavity through body pores.


Animal Physiology

There are several coelenterates which, like sponges, are sessile animals. However, they procure

food by trapping method rather than the current method noted in sponges. The important structure

involved in the trappin mechanism is the cnidoblast (nematocyst). The tentacles in Hydra and some

other coelenterates are armed with cnidoblasts and these would burst when small animals brush or

bump over the cnidocil which is a small hair-like process serving as receptor of contact stimuli. As a

result of this burst a poisonous thread from the nematocysts is thrust into the victim to impale it. The

impaled organism is then carried to the mouth by one or more of the tentacles.

Filter feeding is characteristic of sessile animals and sedentary feeders such as bivalve molluscs,

Amphioxus, Ammocoete larva, etc. These animals have varied types of filtering and trapping devices.

Cilia and setae produce water currents which carry the food particles to the the feeding surfaces.

These particles get entangled in the mucus cord which is carried into the digestive, tract by the aid of

ciliary movement. Certain sea-cucumbers have sticky tentacles which trap small organisms. These

tentacles are then thrust into the pharynx in order to wipe off food from them.

Although filter feeding is a characteristic feature of sessile and sedentary group of animals, it is

also present in a few other animal groups. Small active copepods, sock-eye salmon, the huge basking

shark and the whalebone whale are all filter feeders. The filter feeders do not select their food, hence

they are known as nonselective feeders. However, they respond to certain chemicals and stimuli by

operating, or by preventing the filter mechanism, depending on favourable or hazaradous conditions.

In other nonselective feeders such as many annelids, some echinoderms and hemichordates, food

enters the body by water currents. From this whatever is needed by the body is absorbed and the rest

discarded. The animals of this group are omnivorous provided those which come in their way should

be sufficiently small to be collected in their filters or traps. Thus collected food is subjected in their

filters or traps. The collected food is subjected to grinding when such organs are present. If absent,

the food material is passed directly for chemical digestion.

Selective feeders employ various methods for the capture and effective utilization of bulky foods

or for withdrawing the juices from animals and plants. Each mechanism is supported by suitable

anatomical modifications and physiological alterations. While the fishes, amphibians, reptiles and

many birds swallow their food without chewing, the mammals masticate food and supply it to the

digestive system in the form of small size particles. For efficient mastication, changes have been

brought about in the jaw bones and muscles. The air passages are separated and guarded by soft

epiglottis and pharyngeal folds in order to facilitate breathing during mastication. These changes are

directly related to the changes in the feeding habits.

The dependence of selective feeders on specific types of food is so great that in its absence they

suffer from functional irregularities. The female mosquito, for instance, needs a meal of blood for the

development of eggs. The rabbit flea, Spilopsyllus cuniculi, requires the blood of breeding, or

pregnant rabbit, containing reproductive hormones, to carry out its own reproductive cycle.

Selective feeders have neurosensory and neuromuscular specializations to enable them to locate

and capture specific foods. The neurosensory abilities may be in the development of visual, chemical

and other stimuli.

Tài liệu bạn tìm kiếm đã sẵn sàng tải về

16 β-Oxidation of Fatty Acids

Tải bản đầy đủ ngay(0 tr)